Example of a Research Plan Essay

Statement of the ProblemThe aim of the contem main office is to identify the onus of different pH level of the agar-agar-agar plate to the antibacterial activity of Santol (Sandoricum koet jape). Specific wholey, the theatre of operations leave seek for the answer of the hesitation 1.) Is on that point any release on the No.of colonies after applying Santol (Sandoricum koet jape) extract on the alimentary agar plate with pH level greater than seven (base)? 2.) Is there any difference on the No.of colonies after applying Santol (Sandoricum koet jape) extract on the agar-agar plate with pH level less than seven (acid)? Hypothesis1.) thither is no.difference on the No.of colonies after applying Santol (Sandoricum koet jape) extract on the agar-agar plate with pH level greater than seven (base). 2.) There is difference on the No.of colonies after applying Santol (Sandoricum koet jape) extract on the agar-agar plate with pH level less than seven (acid).Research MethodologyM aterials and EquipmentThe study will make utilisation of Agar Plates, specifically nourishing Agar, for different tests and as a controlled variable for the problem, Nutrient Broth, same purpose as the nutrient agar, Santol (Sandoricum koet jape) extract, Escherichia coli, for the gram positive, Bacillus , for gram negative, and Candida________ a test subject for the study. Also, the study will make use of litmus paper for measuring and maintining the pH level of the Agar plates. The study will also make use of spectrophotometry.Research conceptThe study will use Complete randomized Design as a Research Design. Control Group Design is two parallel experiments are set up, identical in all respects except that only genius includes the treatment being explored by the experiment. The control group may have no treatment, with nothing happening to them, or they may have a neutral treatment.Statistical machineThe study will make use of ANOVA as its statistical tool. T-test kitty be use to compare two dependent samples/matched-pairs picture experiments. The study will make use of two types of pH caustic and basic. Each Agar Plate will be assign to one specific pH level.Inoculate the microbes in the Agar and in the neckcloth. This can be done by streaking the loop containing the gram positive, gram negative, and the kingdom Fungi . Data Gathering ProcedurePrepare the Agar plate and the broth for the experimentation. The pH of the Agar and the broth mustiness also be set. amaze the agar filled petri dishes and the broth that have been inoculated in a dark, fervent (86 F) place for a week for incubation.Apply the extract of Santol (Sandoricum koet jape) to each Agar plate and Nutrient Broth.Using the Colony Formation building block (CFU), contain the number of colony inhibited.Record the data from the Agar plates and the Nutrient broth. figure of speech 3.1 Experimental ProceduresFigure 3.1 Experimental Procedures shows the step by step subroutine of the experiment. Starting from the preparation of the Agar plate with their assign pH level. The Agar plate will solidify at 32C- 40C but will melt at approximately 85C. following is to inoculate the bacteria by streaking the loop containing the bacteria. After inoculating the bacteria, the agar plate must be incubated for one week. After the incubation of the agar plates, apply the Santol (Sandoricum koet jape) extract to the each agar plate. Each plate must receive equal amount of the extract. Using the Colony Formation Unit (CFU), determine the number of inhibited colony/ies and record the data.Data miscellanypH aim of the Agar Plate Number of Colonies Trial 1Mean of three Repetitions Trial 2Mean of leash Repetitions Trial 3Mean of triple Repetitions pH 5 pH 6 pH 7 pH 8 pH 9 Figure 3.2 Interactions in Acidic, Basic, and Neutral Agar Plate Figure 3.2 shows the effect of pH levels less than seven (Acid) on the no. of colonies.pH Level of the Nutrient Broth Number of Col onies Trial 1Mean of Three Repetitions Trial 2Mean of Three Repetitions Trial 3Mean of Three Repetitions pH 1 pH 2 pH 3 pH 4 Figure 3.3 Interactions in Acidic Nutrient BrothShows the effect of pH levels lower than seven (Acid) on the no. of colonies. pH Level of the Nutrient Broth Number of Colonies Trial 1Mean of Three Repetitions Trial 2Mean of Three Repetitions Trial 3Mean of Three Repetitions pH10 pH11 pH12 pH13 pH14 Figure 3.4. Interactions in Basic Nutrient BrothShows the effects of pH levels greater than seven (Basic) on the no. of coloniesBibliography* Anke, T. & Weber, D. (2009). Physiology and Genetics Selected Basic and Applied Aspects, Volume 15. Springer-Verlag Heidelberg, Germany * Chapelle, F. (2001). Ground-water Microbiology and Geochemistry (2nd ed.). John Wiley & Sons, Inc. Canada. * Kubitzki, K. (2011). unfolding Plants. Eudicots Sapindales, Cucurbitals, Myrtaceae. Springer-Verlag Heidelberg, Germany. * Shahidi, F. & Ho, C. T. (2000). Phytochem icals and Phytopharmaceuticals. AOCS Press USA. * Storz, G. & Aronis, R.H. (2000). Bacteria Stress Responses. ASM Press USA. * Agars. Difco & BBL Manual. http//www.bd.com/ds/technicalCenter/inserts/Agars.pdf, accessed January 17, 2008. * Agar Bottles Preparation & Equipment Use. Science Stuff, Inc. http//www.sciencestuff.com/ vacation spot/agar_bottle.shtml, accessed January 14, 2005. Mott, et al. * Agar Bottles Preparation & Equipment Use. 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